Phospho-PKC Alpha (Thr638) Recombinant antibody

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  WB analysis using 81575-2-RR

  Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-PKC Alpha (Thr638) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 81575-2-RR

Clone No.241844A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-PKC α Thr638, Phospho-PKC beta (Thr641), Phospho-PKC, p-PKC, p-PKC Alpha

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Tested Applications

• Positive WB detected in: THP-1 cells, λ phosphatase treated THP-1 cells

The antibody recognizes PKC Beta-II protein phosphorylated at Thr641.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81575-2-RR targets Phospho-PKC Alpha (Thr638) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein kinase C, alpha
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: AK055431
• Gene Symbol: PKC Alpha
• Gene ID (NCBI): 5578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P17252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)