Phospho-P53 (Ser15) Recombinant antibody, PBS Only

Phospho-P53 (Ser15) Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2J21

Cat no : 80195-1-PBS

Synonyms

TP53, P53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

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Validation Data Gallery

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  WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

  Non-treated and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  WB analysis using 80195-1-RR (same clone as 80195-1-PBS)

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80195-1-RR (Phospho-P53 (Ser15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 80195-1-RR (same clone as 80195-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed etoposide treated HT-29 cells and non-treated HT-29 cells using Phospho-P53 (Ser15) antibody (80195-1-RR, Clone: 2J21 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 80195-1-RR (same clone as 80195-1-PBS)

  1X10^6 A431 cells untreated (dashed line) or treated with UV (red) were intracellularly stained with 0.13 ug Anti-Human Phospho-P53 (Ser15) (80195-1-RR, Clone:2J21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80195-1-PBS in a different storage buffer formulation.

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Product Information

80195-1-PBS targets Phospho-P53 (Ser15) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC003596
• Gene Symbol: P53
• Gene ID (NCBI): 7157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)