Phospho-P53 (Ser15) Recombinant antibody
Phospho-P53 (Ser15) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2J21
Cat no : 80195-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | etoposide treated HT-29 cells, UV treated A431 cells |
Positive IF/ICC detected in | etoposide treated HT-29 cells |
Positive FC (Intra) detected in | UV treated A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
80195-1-RR targets Phospho-P53 (Ser15) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | tumor protein p53 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 53 kDa |
GenBank Accession Number | BC003596 |
Gene Symbol | P53 |
Gene ID (NCBI) | 7157 |
RRID | AB_2918873 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)
Protocols
Product Specific Protocols | |
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WB protocol for Phospho-P53 (Ser15) antibody 80195-1-RR | Download protocol |
IF protocol for Phospho-P53 (Ser15) antibody 80195-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Rep Involvement of JNK signaling in Aspergillus fumigatus-induced inflammatory factors release in bronchial epithelial cells | ||
Cells Concurrent Activation of Both Survival-Promoting and Death-Inducing Signaling by Chloroquine in Glioblastoma Stem Cells: Implications for Potential Risks and Benefits of Using Chloroquine as Radiosensitizer |