Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody

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• Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody (82335-1-RR)

Phospho-NF-κB p65 (Ser468) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA, ChIP-qPCR

Cat No. 82335-1-RR Copied!

Clone No. 6N1

Publications (631)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF, FC (Intra), ELISA, ChIP-qPCR

Synonyms

NFκB (Ser468), NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468)

• 

  WB analysis using 82335-1-RR

  Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of NIH/3T3 using 82335-1-RR

  1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-NF-κB p65 (Ser468) Recombinant antibody (82335-1-RR, Clone:6N1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of HeLa using 82335-1-RR

  Chromatin was prepared from HeLa cells treated with hTNF-α (30 ng/ml, 1 h). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-NF-κB p65 (Ser468) (82335-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells
• Positive FC (Intra) detected in: Calyculin A treated NIH/3T3 cells
• Positive ChIP-qPCR detected in: hTNF-α (30 ng/ml, 1 h) HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82335-1-RR targets Phospho-NF-κB p65 (Ser468) in WB, IHC, IF, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC011603
• Gene Symbol: NF-κB p65
• Gene ID (NCBI): 5970
• RRID: AB_3083091
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q04206
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

Publications

What published studies show

The antibody is Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody (6N1), catalog number 82335-1-RR, with a total of 631 citations. Citations span high-impact journals including Nature Immunology, Nature Communications, Nature Microbiology, Cancer Research, Journal of Clinical Investigation, Advanced Science, Biomaterials, Cell Death & Differentiation, Science Advances, Theranostics, Phytomedicine, and others. Associated research fields include inflammation/NF-κB signaling, oncology, immunology, neuroscience, nanomedicine/drug delivery, gut microbiota, cardiovascular disease, liver/kidney injury, bone/joint disorders, sepsis, pulmonary disease, diabetes, and phytochemistry.

Automatically generated from the 631 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Human	WB	Signal Transduct Target Ther; Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells; Authors - Pengju Wang
mouse	WB	Signal Transduct Target Ther; Innovative peptide-loaded hybrid membrane nanovaccine enables precise personalized immunotherapy for HPV-related cervical diseases.; Authors - Renjie Wang
human, mouse	WB,IF	Exploration (Beijing); Caffeic Acid Acts as a Potent Senomorphic and Alleviates Inflammation and Lung Fibrosis by Covalently Targeting Annexin A5 Protein in Mice.; Authors - Yinhua Zhu
human,mouse	WB	Nat Immunol; Sparcl1 mitigates abdominal aortic aneurysm through inhibiting lymphangiogenesis-mediated TLS formation; Authors - Mei-Hua Chen
		Bioact Mater; Integrated apoptotic extracellular vesicle-recruitment peptide coating reprograms the diabetic bone microenvironment and orchestrates enhanced implant osseointegration.; Authors - Jining Shen
human	WB	Cancer Res; The Glycosyltransferase XYLT1 Activates NF-κB Signaling to Promote Metastasis of Early-Stage Lung Adenocarcinoma; Authors - Jian Han