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Phospho-NF-κB p65 (Ser468) Recombinant antibody, PBS Only

Phospho-NF-κB p65 (Ser468) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6N1

Cat no : 82335-1-PBS

Synonyms

NFKB3, p65, Phospho-NF-κB p65 (Ser468), RELA, Transcription factor p65

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-NF-κB p65 (Ser468) Recombinant antibody (82335-1-RR)

    WB analysis using 82335-1-RR (same clone as 82335-1-PBS)

    Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently. This data was developed using the same antibody clone with 82335-1-PBS in a different storage buffer formulation.

Product Information

82335-1-PBS targets Phospho-NF-κB p65 (Ser468) in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC011603
Gene Symbol NF-κB p65
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.