CoraLite® Plus 647-conjugated Phospho-MYL9 (Thr19/Ser20) Recombinant antibody
Phospho-MYL9 (Thr19/Ser20) Uni-rAbTM Recombinant Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1G10
Cat no : CL647-82717
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
This antibody, also known as Thr18/Ser19, is the site where the initiator methionine is removed.
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-82717 targets Phospho-MYL9 (Thr19/Ser20) in FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | myosin, light chain 9, regulatory |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 19-20 kDa |
| GenBank Accession Number | BC002648 |
| Gene Symbol | MYL9 |
| Gene ID (NCBI) | 10398 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).
