Phospho-JNK (Tyr185) Recombinant antibody, PBS Only

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  WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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  WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

  Non-treated and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80024-1-RR (same clone as 80024-1-PBS)

  Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 80024-1-RR (Phospho-JNK (Tyr185) antibody) at dilution of 1:2600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 80024-1-RR (same clone as 80024-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-JNK (Tyr185) Recombinant antibody (80024-1-RR, Clone:2G8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80024-1-PBS in a different storage buffer formulation.

Phospho-JNK (Tyr185) Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 80024-1-PBS

Clone No.2G8

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24

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Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

80024-1-PBS targets Phospho-JNK (Tyr185) in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 8
• Observed Molecular Weight: 37-46 kDa
• GenBank Accession Number: NM_139049
• Gene Symbol: JNK
• Gene ID (NCBI): 5599
• RRID: AB_2882943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P45983
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)

Publications

Species	Application	Title
human,rat	WB,IHC	Redox Rep; Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells; Authors - Xiaozhe Su