Tested Applications
| Positive WB detected in | HEK-293T cells, Calyculin A treated HEK-293T cells, UV treated HEK-293T cells, UV treated NIH/3T3 cells, Anisomycin treated NIH/3T3 cells |
| Positive IF/ICC detected in | UV treated HEK-293 cells |
| Positive FC (Intra) detected in | Calyculin A treated HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80024-1-RR targets Phospho-JNK (Tyr185) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase 8 |
| Observed Molecular Weight | 37-46 kDa |
| GenBank Accession Number | NM_139049 |
| Gene Symbol | JNK |
| Gene ID (NCBI) | 5599 |
| RRID | AB_2882943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P45983 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. In the phosphorylation of JNK, JNK1 and JNK2/3 have molecular weights of 46 and 54 kDa(PMID: 21378396)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Download protocol |
| WB protocol for Phospho-JNK (Tyr185) antibody 80024-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-JNK (Tyr185) Recombinant monoclonal antibody (2G8), catalog number 80024-1-RR, has accumulated 466 citations. These publications appear in high-impact journals including Nature Communications, Molecular Cancer, Cell Metabolism, Advanced Science, Journal of Nanobiotechnology, and Pharmacological Research, among many others. The cited research spans diverse fields such as oncology, neuroinflammation, cardiovascular disease, metabolic disorders, immunology, bone biology, fibrosis, and toxicology, with Western blot being the predominant application.
| Species | Application | Title |
|---|---|---|
Mol Cancer Targeting BATF2-RGS2 axis reduces T-cell exhaustion and restores anti-tumor immunity | ||
J Hepatol Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD | ||
Cell Metab Remote limb ischemic conditioning alleviates steatohepatitis via extracellular vesicle-mediated muscle-liver crosstalk | ||
Mil Med Res D-mannose alleviates intervertebral disc degeneration through glutamine metabolism | ||
Cancer Commun (Lond) Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma | ||
J Extracell Vesicles S100A9 Aggravates Cardiac Fibrosis by TLR4/PGC-1α Mediated Macrophage-Myofibroblast Crosstalk. |
