CoraLite® Plus 488-conjugated Phospho-EIF2S1 (Ser51) Monoclonal antibody

Phospho-EIF2S1 (Ser51) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

1

CloneNo.

1A4A11

Cat no : CL488-68023

Synonyms

EIF2S1, EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-68023

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-EIF2S1 (Ser51) antibody (CL488-68023, Clone: 1A4A11 ) at dilution of 1:200.

• 

  FC experiment of PC-3 using CL488-68023

  1x10^6 Calyculin A treated PC-3 cells were intracellularly stained with 0.13 ug CoraLite® Plus 488-conjugated Phospho-EIF2S1 (Ser51) Monoclonal antibody (CL488-68023, Clone:1A4A11)(red), or 0.13 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Tested Applications

• Positive IF/ICC detected in: Calyculin A treated HeLa cells
• Positive FC (Intra) detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL488-68023 targets Phospho-EIF2S1 (Ser51) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: NM_004094
• Gene Symbol: EIF2S1
• Gene ID (NCBI): 1965
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Publications

Species	Application	Title
	IF	Nat Commun; LNP-RNA-engineered adipose stem cells for accelerated diabetic wound healing; Authors - Yonger Xue