Phospho-EIF2S1 (Ser51) Monoclonal antibody

Phospho-EIF2S1 (Ser51) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A4A11

Cat no : 68023-1-Ig

Synonyms

EIF2S1, EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, Calyculin A treated HEK-293 cells, NIH/3T3 cells, HSC-T6 cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells
Positive IF/ICC detected inCalyculin A treated HeLa cells
Positive FC (Intra) detected inCalyculin A treated PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

68023-1-Ig targets Phospho-EIF2S1 (Ser51) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Calculated Molecular Weight 36 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberNM_004094
Gene Symbol EIF2S1
Gene ID (NCBI) 1965
RRIDAB_2918767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Protocols

Product Specific Protocols
WB protocol for Phospho-EIF2S1 (Ser51) antibody 68023-1-IgDownload protocol
IF protocol for Phospho-EIF2S1 (Ser51) antibody 68023-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Mitosis exit followed by death in interphase prevents the development of polyploid giant cancer cells

Authors - Juan Jesus Vicente
humanWB

Cell Death Dis

AURKA emerges as a vulnerable target for KEAP1-deficient non-small cell lung cancer by activation of asparagine synthesis

Authors - Bing Deng
humanWB

J Transl Med

The microtubule targeting agent ST-401 triggers cell death in interphase and prevents the formation of polyploid giant cancer cells

Authors - Juan Jesus Vicente
humanWB

Environ Pollut

Copper exposure induces mitochondrial dysfunction and hepatotoxicity via the induction of oxidative stress and PERK/ATF4 -mediated endoplasmic reticulum stress

Authors - Meng Li
human,mouseWB

Microbiol Res

Berberine ameliorates vascular dysfunction by downregulating TMAO-endoplasmic reticulum stress pathway via gut microbiota in hypertension

Authors - Zhichao Wang
humanWB

Eur J Med Chem

Discovery of novel quinoline scaffold selective estrogen receptor degraders (SERDs) for treatment of ER positive breast cancer with enhanced antiproliferative bioactivity through immunogenic cell death (ICD) effects

Authors - Yunlong Lu