CoraLite®555-conjugated Palladin Polyclonal antibody

Palladin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-10853

Synonyms

PALLD,palladin, PALLD, PNCA1, KIAA0992, CGI-151

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Validation Data Gallery

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  IF Staining of mouse testis using CL555-10853

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 Palladin antibody (CL555-10853) at dilution of 1:200.

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  IF Staining of mouse testis using CL555-10853

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®555 Palladin antibody (CL555-10853) at dilution of 1:200.

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  FC experiment of HeLa using CL555-10853

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Palladin (CL555-10853) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: mouse testis tissue
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-10853 targets Palladin in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Palladin fusion protein Ag1288
• Full Name: palladin, cytoskeletal associated protein
• Calculated Molecular Weight: 1383 aa, 151 kDa
• Observed Molecular Weight: 95 kDa, 140 kDa
• GenBank Accession Number: BC013867
• Gene Symbol: palladin
• Gene ID (NCBI): 23022
• RRID: AB_2919594
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C