Palladin Polyclonal antibody

• Featured Product
• KD/KO Validated

Palladin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, COIP, ELISA

Conjugate

Unconjugated

Publications

72

Cat no : 10853-1-AP

Synonyms

PALLD,palladin, PALLD, PNCA1, KIAA0992, CGI-151

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Validation Data Gallery

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  WB analysis of A549 cells using 10853-1-AP

  A549 cells (shcontrol and shRNA of PALLD) were subjected to SDS PAGE followed by western blot with 10853-1-AP (PALLD antibody) at dilution of 1:2000.

• 

  WB analysis of HeLa using 10853-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10853-1-AP (Palladin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10853-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10853-1-AP (Palladin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10853-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10853-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10853-1-AP (Palladin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10853-1-AP

  IP result of anti-Palladin (IP:10853-1-AP, 4ug; Detection:10853-1-AP 1:8000) with HeLa cells lysate 1280 ug.

• 

  IP experiment of HEK-293 using 10853-1-AP

  IP result of anti-Palladin (IP:10853-1-AP, 4ug; Detection:10853-1-AP 1:300) with HEK-293 cells lysate 1500ug.

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10853-1-AP (Palladin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10853-1-AP (Palladin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10853-1-AP (Palladin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10853-1-AP (Palladin antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 10853-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Palladin antibody (10853-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 10853-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Palladin Polyclonal antibody (10853-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IP detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human colon tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 56 publications below
• IHC: See 12 publications below
• IF: See 43 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

10853-1-AP targets Palladin in WB, IHC, IF/ICC, FC (Intra), IP, COIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Palladin fusion protein Ag1288
• Full Name: palladin, cytoskeletal associated protein
• Calculated Molecular Weight: 1383 aa, 151 kDa
• Observed Molecular Weight: 95 kDa, 140 kDa
• GenBank Accession Number: BC013867
• Gene Symbol: palladin
• Gene ID (NCBI): 23022
• RRID: AB_2158782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.

Publications

Species	Application	Title
human	IP	Mol Cell; The actin-bundling protein palladin is an Akt1-specific substrate that regulates breast cancer cell migration.; Authors - Chin Y Rebecca YR
human, mouse	WB,IHC	Nat Commun; Local microRNA delivery targets Palladin and prevents metastatic breast cancer.; Authors - Avital Gilam ; KD Validated
human	WB	Cell Death Differ; lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus.; Authors - Hai-Ting Liu
human	IF	J Am Soc Nephrol; The Role of Palladin in Podocytes.; Authors - Nadine Artelt
human	IHC	Cancer Res; Cofilin Drives Cell Invasive and Metastatic Responses to TGF-β in Prostate Cancer.; Authors - Joanne Collazo
human	WB,IHC,IF	Oncogene; Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).; Authors - I García-Palmero ; KD Validated

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