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- KD/KO Validated
Palladin Polyclonal antibody
Palladin Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 10853-1-AP
Validation Data Gallery
|Positive WB detected in
|HeLa cells, A549 cells
|Positive IP detected in
|HeLa cells, HEK-293 cells
|Positive IHC detected in
|human colon cancer tissue, human breast cancer tissue, human colon tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:20-1:200
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10853-1-AP targets Palladin in WB, IP, IHC, IF, FC, COIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Palladin fusion protein Ag1288
|palladin, cytoskeletal associated protein
|Calculated molecular weight
|1383 aa, 151 kDa
|Observed molecular weight
|95 kDa, 140 kDa
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.
|Product Specific Protocols
|WB protocol for Palladin antibody 10853-1-AP
|IHC protocol for Palladin antibody 10853-1-AP
|IF protocol for Palladin antibody 10853-1-AP
|IP protocol for Palladin antibody 10853-1-AP
|FC protocol for Palladin antibody 10853-1-AP
|Click here to view our Standard Protocols
The actin-bundling protein palladin is an Akt1-specific substrate that regulates breast cancer cell migration.
Local microRNA delivery targets Palladin and prevents metastatic breast cancer.
Cell Death Differ
lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus.
J Am Soc Nephrol
The Role of Palladin in Podocytes.
Cofilin Drives Cell Invasive and Metastatic Responses to TGF-β in Prostate Cancer.
Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).