PTPN12 Recombinant antibody

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  WB analysis using 83851-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83851-6-RR (PTPN12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 83851-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83851-6-RR (PTPN12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83851-6-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U-251 using 83851-6-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83851-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83851-6-RR against Human PTPN12 were performed. The affinity constant is 48.4 pM.

PTPN12 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83851-6-RR

Clone No.240983C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTPG1, PTP PEST, 240983C9

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, HEK-293T cells, A549 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83851-6-RR targets PTPN12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTPN12 fusion protein Ag35382
• Full Name: protein tyrosine phosphatase, non-receptor type 12
• Calculated Molecular Weight: 88kDa
• Observed Molecular Weight: 110-125 kDa
• GenBank Accession Number: NM_002835.3
• Gene Symbol: PTPN12
• Gene ID (NCBI): 5782
• RRID: AB_3671436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q05209
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.