PTPN12 Recombinant antibody, PBS Only

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  WB analysis using 83851-6-RR (same clone as 83851-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83851-6-RR (PTPN12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83851-6-RR (same clone as 83851-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83851-6-RR (PTPN12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83851-6-RR (same clone as 83851-6-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 83851-6-RR (same clone as 83851-6-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83851-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83851-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83851-6-RR against Human PTPN12 were performed. The affinity constant is 48.4 pM.

PTPN12 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 83851-6-PBS

Clone No.240983C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PTPG1, PTP PEST, 240983C9

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Product Information

83851-6-PBS targets PTPN12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTPN12 fusion protein Ag35382
• Full Name: protein tyrosine phosphatase, non-receptor type 12
• Calculated Molecular Weight: 88kDa
• Observed Molecular Weight: 110-125 kDa
• GenBank Accession Number: NM_002835.3
• Gene Symbol: PTPN12
• Gene ID (NCBI): 5782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q05209
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.