GINS2 Recombinant antibody

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  WB analysis using 84486-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84486-2-RR (GINS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84486-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GINS2 antibody (84486-2-RR, Clone: 241870E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 84486-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Recombinant antibody (84486-2-RR, Clone:241870E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84486-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84486-2-RR against Human GINS2 were performed. The affinity constant is 0.82 nM.

GINS2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84486-2-RR

Clone No.241870E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PSF2, Pfs2, HSPC037, GINS 2, DC5

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84486-2-RR targets GINS2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GINS2 fusion protein Ag9180
• Full Name: GINS complex subunit 2 (Psf2 homolog)
• Calculated Molecular Weight: 185 aa, 21 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC010164
• Gene Symbol: GINS2
• Gene ID (NCBI): 51659
• RRID: AB_3672003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y248
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.