PRDM8 Recombinant antibody, PBS Only (Capture)

PRDM8 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242203D8

Cat no : 84716-2-PBS

Synonyms

PR domain-containing protein 8, PR domain containing protein 8, PR domain containing 8, EC:2.1.1.-, 242203D8

---

Validation Data Gallery

• 

  WB analysis using 84716-2-RR (same clone as 84716-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse eye using 84716-2-RR (same clone as 84716-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84716-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01532-1

  Cytometric bead array standard curve of MP01532-1, PRDM8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84716-2-PBS. Detection antibody: 84716-3-PBS. Standard: Ag23373. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84716-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

---

Product Information

84716-2-PBS targets PRDM8 as part of a matched antibody pair:

MP01532-1: 84716-2-PBS capture and 84716-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRDM8 fusion protein Ag23373
• Full Name: PR domain containing 8
• GenBank Accession Number: BC071584
• Gene Symbol: PRDM8
• Gene ID (NCBI): 56978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).