PRDM8 Recombinant antibody

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  WB analysis using 84716-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 84716-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 84716-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 84716-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 84716-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84716-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

PRDM8 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 84716-2-RR

Clone No.242203D8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

PR domain-containing protein 8, PR domain containing protein 8, PR domain containing 8, EC:2.1.1.-, 242203D8

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84716-2-RR targets PRDM8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PRDM8 fusion protein Ag23373
• Full Name: PR domain containing 8
• GenBank Accession Number: BC071584
• Gene Symbol: PRDM8
• Gene ID (NCBI): 56978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQV8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).