• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations
Filter:
  • Western Blot (WB) analysis of various lysates using PRDM8 Recombinant antibody (84716-2-RR)

    WB analysis using 84716-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IHC staining of mouse eye using 84716-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IHC staining of mouse eye using 84716-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IF Staining of mouse eye using 84716-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IF Staining of mouse eye using 84716-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84716-2-RR

    Affinity and Kinetic Characterization of 84716-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

PRDM8 Recombinant monoclonal antibody

PRDM8 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84716-2-RR
Clone No.242203D8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

242203D8, EC:2.1.1.-, PR domain containing 8, PR domain containing protein 8, PR domain-containing protein 8

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84716-2-RR targets PRDM8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag23373

Product name: Recombinant human PRDM8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC071584

Sequence: MEDTGIQRGIWDGDAKAVQQCLTDIFTSVYTTCDIPENAIFGPCVLSHTSLYDSIAFIALKSTDKRTVPYIFRVDTSAANGSSEGLMWLRLVQSARDKEEQNLEAYIKNGQLFYRSLRRIAKDEELLVWYGKELTELLLLCPSRSHNKMNGSSPYTCLECSQRFQFEFPYVAHLRFRCPKRLHSADISPQDEQGGGVGTKDHGGGGGGGKDQQQQQQEAPLGPGPKFCKAGPLHHYPSPSPESSNPSAAAGGSSAKPSTDFHNLARELENSRGGSSCSPAQSLSSGSGSGGGGGHQEAELSPDGIATGGGKGKRKFPEEAAEGGGGAGLVGGRGRFVERPLPASKEDLVC

Predict reactive species
Full Name PR domain containing 8
Calculated Molecular Weight 689 aa, 72 kDa
Observed Molecular Weight72 kDa
GenBank Accession NumberBC071584
Gene Symbol PRDM8
Gene ID (NCBI) 56978
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NQV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).

Protocols

Product Specific Protocols
IF protocol for PRDM8 antibody 84716-2-RRDownload protocol
IHC protocol for PRDM8 antibody 84716-2-RRDownload protocol
WB protocol for PRDM8 antibody 84716-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...