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PRDM8 Recombinant antibody

PRDM8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

242203D8

Cat no : 84716-2-RR

Synonyms

PR domain-containing protein 8, PR domain containing protein 8, PR domain containing 8, EC:2.1.1.-, 242203D8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRDM8 Recombinant antibody (84716-2-RR)

    WB analysis using 84716-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84716-2-RR (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IHC staining of mouse eye using 84716-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IHC staining of mouse eye using 84716-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 84716-2-RR (PRDM8 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IF Staining of mouse eye using 84716-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRDM8 Recombinant antibody (84716-2-RR)

    IF Staining of mouse eye using 84716-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRDM8 antibody (84716-2-RR, Clone: 242203D8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84716-2-RR

    Affinity and Kinetic Characterization of 84716-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84716-2-RR against Human PRDM8 were performed. The affinity constant is 31.8 nM.

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84716-2-RR targets PRDM8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRDM8 fusion protein Ag23373
Full Name PR domain containing 8
GenBank Accession NumberBC071584
Gene Symbol PRDM8
Gene ID (NCBI) 56978
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).

Protocols

Product Specific Protocols
WB protocol for PRDM8 antibody 84716-2-RRDownload protocol
IHC protocol for PRDM8 antibody 84716-2-RRDownload protocol
IF protocol for PRDM8 antibody 84716-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols