CoraLite®594-conjugated PRC1 Monoclonal antibody

PRC1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E1D5

Cat no : CL594-67027

Synonyms

ASE1, PRC1

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67027

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PRC1 antibody (CL594-67027, Clone: 1E1D5 ) at dilution of 1:100.

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  IF Staining of HeLa using CL594-67027

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 PRC1 antibody (CL594-67027, Clone: 1E1D5 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67027 targets PRC1 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRC1 fusion protein Ag5907
• Full Name: protein regulator of cytokinesis 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC003138
• Gene Symbol: PRC1
• Gene ID (NCBI): 9055
• RRID: AB_2920075
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.