PRC1 Monoclonal antibody

• Featured Product
• KD/KO Validated

PRC1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1D5

Cat no : 67027-1-Ig

Synonyms

ASE1, PRC1

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Validation Data Gallery

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  WB analysis using 67027-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67027-1-Ig (PRC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67027-1-Ig

  WB result of PRC1 antibody (67027-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

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  IHC staining of human breast cancer using 67027-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67027-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67027-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67027-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67027-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67027-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, HepG2 cells, HSC-T6 cells, 4T1 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67027-1-Ig targets PRC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRC1 fusion protein Ag5907
• Full Name: protein regulator of cytokinesis 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC003138
• Gene Symbol: PRC1
• Gene ID (NCBI): 9055
• RRID: AB_2882342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.