PRC1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PRC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1D5

Cat no : 67027-1-PBS

Synonyms

ASE1, PRC1

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Validation Data Gallery

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  WB analysis using 67027-1-Ig (same clone as 67027-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67027-1-Ig (PRC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67027-1-Ig (same clone as 67027-1-PBS)

  WB result of PRC1 antibody (67027-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67027-1-Ig (PRC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67027-1-Ig (same clone as 67027-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRC1 antibody (67027-1-Ig, Clone: 1E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67027-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67027-1-PBS targets PRC1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRC1 fusion protein Ag5907
• Full Name: protein regulator of cytokinesis 1
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC003138
• Gene Symbol: PRC1
• Gene ID (NCBI): 9055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.