Tested Applications
Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-66300 targets Progesterone Receptor in IHC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Progesterone Receptor fusion protein Ag22992 Predict reactive species |
Full Name | progesterone receptor |
Calculated Molecular Weight | 933 aa, 99 kDa |
GenBank Accession Number | NM_000926 |
Gene Symbol | Progesterone Receptor |
Gene ID (NCBI) | 5241 |
RRID | AB_2883069 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P06401 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin Progesterone Receptor antibody Biotin-66300 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |