CoraLite® Plus 488-conjugated PPP2R2A/B/C Monoclonal antibody

PPP2R2A/B/C Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E1D5

Cat no : CL488-67783

Synonyms

PPP2R2B, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA, B55β

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67783

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PPP2R2B (CL488-67783, Clone:2E1D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67783 targets PPP2R2A/B/C in FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PPP2R2A/B/C fusion protein Ag30774
• Full Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Calculated Molecular Weight: 443 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC031790
• Gene Symbol: PPP2R2B
• Gene ID (NCBI): 5521
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C