CoraLite® Plus 488-conjugated POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15278

Synonyms

Arsenic transactivated protein, AsTP, CHARAC17, CHRAC 17, CHRAC17, DNA polymerase II subunit 3, HuCHRAC17, p17, POLE3, YBL1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-15278

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 POLE3 antibody (CL488-15278) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15278 targets POLE3 in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLE3 fusion protein Ag7396
• Full Name: polymerase (DNA directed), epsilon 3 (p17 subunit)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC003166
• Gene Symbol: POLE3
• Gene ID (NCBI): 54107
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.