POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15278-1-AP

Synonyms

CHRAC17, CHRAC 17, CHARAC17, AsTP, Arsenic-transactivated protein

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Validation Data Gallery

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  WB analysis using 15278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15278-1-AP (POLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15278-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15278-1-AP (POLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15278-1-AP

  IP result of anti-POLE3 (IP:15278-1-AP, 4ug; Detection:15278-1-AP 1:1000) with HepG2 cells lysate 1800ug.

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  IHC staining of human liver cancer using 15278-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 15278-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 15278-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15278-1-AP (POLE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15278-1-AP targets POLE3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLE3 fusion protein Ag7396
• Full Name: polymerase (DNA directed), epsilon 3 (p17 subunit)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC003166
• Gene Symbol: POLE3
• Gene ID (NCBI): 54107
• RRID: AB_2166751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Biochem Cell Biol; SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.; Authors - Chuanyou Yi
human	WB	Front Oncol; ASF1B Promotes Oncogenesis in Lung Adenocarcinoma and Other Cancer Types.; Authors - Wencheng Zhang