PMS2 Recombinant antibody

• 

  WB analysis using 84894-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 84894-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 84894-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 84894-5-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 84894-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

PMS2 {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 84894-5-RR

Clone No.242455F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, PC-3 cells, MCF-7 cells, A431 cells, Caco-2 cells,
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84894-5-RR targets PMS2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PMS2 fusion protein Ag33508
• Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Calculated Molecular Weight: 862 aa, 96 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC093921
• Gene Symbol: PMS2
• Gene ID (NCBI): 5395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P54278
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).