PMS2 Recombinant antibody, PBS Only

• 

  WB analysis using 84894-5-RR (same clone as 84894-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84894-5-RR (PMS2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84894-5-RR (same clone as 84894-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84894-5-RR (PMS2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 84894-5-RR (same clone as 84894-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PMS2 Recombinant antibody (84894-5-RR, Clone:242455F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84894-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84894-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84894-5-RR against Human PMS2 were performed. The affinity constant is below 1 pM.

PMS2 {ptg:Brand} Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 84894-5-PBS

Clone No.242455F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

PMSL2, PMS2CL, PMS1 protein homolog 2, HNPCC4, EC:3.1.-.-

---

Product Information

84894-5-PBS targets PMS2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PMS2 fusion protein Ag33508
• Full Name: PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
• Calculated Molecular Weight: 862 aa, 96 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: BC093921
• Gene Symbol: PMS2
• Gene ID (NCBI): 5395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P54278
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PMS2, also named as PMSL2, belongs to the DNA mismatch repair mutL/hexB family. It is a component of the post-replicative DNA mismatch repair system (MMR). It heterodimerizes with MLH1 to form MutL alpha. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. It also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. (PMID: 16873062, PMID: 18206974) Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4). Defects in PMS2 are a cause of mismatch repair cancer syndrome (MMRCS).