CoraLite® Plus 488-conjugated PLOD3 Monoclonal antibody

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  IF Staining of HeLa using CL488-60058

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PLOD3 antibody (CL488-60058, Clone: 2G12B8 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

PLOD3 Monoclonal Antibody for IF/ICC

Cat No. CL488-60058

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3, Galactosylhydroxylysine-glucosyltransferase

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60058 targets PLOD3 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLOD3 fusion protein Ag1480
• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Calculated Molecular Weight: 738 aa, 85 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC011674
• Gene Symbol: PLOD3
• Gene ID (NCBI): 8985
• RRID: AB_2883092
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60568
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.