PLOD3 Monoclonal antibody

• Featured Product
• KD/KO Validated

PLOD3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2G12B8

Cat no : 60058-1-Ig

Synonyms

Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Lysyl hydroxylase 3, LH3, Galactosylhydroxylysine-glucosyltransferase

---

Validation Data Gallery

• 

  WB analysis using 60058-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of human placenta using 60058-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60058-1-Ig

  WB result of PLOD3 antibody (60058-1-Ig, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells.

• 

  WB analysis using 60058-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60058-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60058-1-Ig

  IP result of anti-PLOD3 (IP:60058-1-Ig, 5ug; Detection:60058-1-Ig 1:500) with HeLa cells lysate 1400ug.

• 

  IHC staining of human pancreas cancer using 60058-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 60058-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 60058-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD3 antibody (60058-1-Ig, Clone: 2G12B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60058-1-Ig

  Immunofluorescent analysis of HeLa cells using 60058-1-Ig (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

---

Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, human placenta tissue, PC-3 cells, K-562 cells, LNCaP cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

60058-1-Ig targets PLOD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLOD3 fusion protein Ag1480
• Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
• Calculated Molecular Weight: 738 aa, 85 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC011674
• Gene Symbol: PLOD3
• Gene ID (NCBI): 8985
• RRID: AB_2165779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

Publications

Species	Application	Title
human	WB	PLoS One; Lysyl Hydroxylase 3 Localizes to Epidermal Basement Membrane and Is Reduced in Patients with Recessive Dystrophic Epidermolysis Bullosa.; Authors - Stephen A Watt
mouse	WB	Matrix Biol Plus; Use of osteoblast-derived matrix to assess the influence of collagen modifications on cancer cells.; Authors - Neus Bota-Rabassedas
mouse	WB	JCI Insight; Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis.; Authors - Xiaochao Tan