Plasminogen Monoclonal antibody, PBS Only

Plasminogen Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A11

Cat no : 66399-1-PBS

Synonyms

Angiostatin, DKFZp779M0222, plasminogen, PLG

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Validation Data Gallery

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  WB analysis of human plasma using 66399-1-Ig (same clone as 66399-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66399-1-Ig (PLG Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66399-1-PBS targets Plasminogen in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasminogen fusion protein Ag9938
• Full Name: plasminogen
• Calculated Molecular Weight: 810 aa, 91 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC060513
• Gene Symbol: PLG
• Gene ID (NCBI): 5340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.