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Biotin-conjugated PKM1-specific Polyclonal antibody
PKM1-specific Polyclonal Antibody for IHC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IHC
Conjugate
Biotin
Cat no : Biotin-15821
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse brain tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
Biotin-15821 targets PKM1-specific in IHC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | PKM pyruvate kinase, muscle |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | NM_182470 |
Gene Symbol | PKM |
Gene ID (NCBI) | 5315 |
RRID | AB_2883058 |
Conjugate | Biotin |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is conjugated with Biotin and it's specific to PKM1 isoform.
Protocols
Product Specific Protocols | |
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IHC protocol for Biotin PKM1-specific antibody Biotin-15821 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |