PKM1-specific Polyclonal antibody

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  WB analysis using 15821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15821-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15821-1-AP

  rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15821-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15821-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells/tissue using 15821-1-AP

  WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000.

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  WB analysis of mouse muscle/liver tissues using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).

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  WB analysis of mouse heart tissue using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of 293 cell using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control).

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  WB analysis of HepG2/MCF7 cells using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

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  IP experiment of mouse brain using 15821-1-AP

  IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skeletal muscle using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PKM1-specific Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15821-1-AP

Publications(85)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissues, mouse heart tissue, 293 cell, HepG2/MCF7 cells, mouse colon tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human skeletal muscle tissue, human brain tissue, human heart tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 75 publications below
• IHC: See 12 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

15821-1-AP targets PKM1-specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_182470
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• RRID: AB_2163820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14618
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nature; The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.; Authors - Christofk Heather R HR ; KD Validated
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Sci Transl Med; ATP citrate lyase drives vascular remodeling in systemic and pulmonary vascular diseases through metabolic and epigenetic changes; Authors - Yann Grobs
human	WB,CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB	Nat Commun; The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.; Authors - Fang Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Pallavi (Verified Customer) (03-31-2025)

I used 1:5000 anti-PKM1 15821-1-AP, it worked perfectly. The reviews were quite helpful to decide which antibody to buy. The delivery was on time. Just make sure the delivery company hands over the package to you. so better to keep a track of the package.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: purified protein