Filter: AllWBIPIHC

WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of multi-cells/tissue using 15821-1-AP (same clone as 15821-1-PBS)
WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse muscle/liver tissues using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of mouse heart tissue using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis using 15821-1-AP (same clone as 15821-1-PBS)
rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of 293 cell using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
WB analysis of HepG2/MCF7 cells using 15821-1-AP (same clone as 15821-1-PBS)
WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 15821-1-AP (same clone as 15821-1-PBS)
IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 15821-1-AP (same clone as 15821-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15821-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PKM1-specific Polyclonal antibody, PBS Only

PKM1-specific Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15821-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PKM, Pyruvate kinase PKM, OIP 3, EC:2.7.11.1, EC:2.7.10.2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

15821-1-PBS targets PKM1-specific in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	PKM pyruvate kinase, muscle
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	NM_182470
Gene Symbol	PKM
Gene ID (NCBI)	5315
RRID	AB_2163820
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14618
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.