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PKM1-specific Polyclonal antibody

Name: PKM1-specific Polyclonal antibody
Brand: Proteintech
SKU: 15821-1-AP

PKM1-specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15821-1-AP

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM1-specific, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

Validation Data Gallery

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AllWBIPIHC

WB analysis using 15821-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 15821-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 15821-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 15821-1-AP
rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of multi-cells/tissue using 15821-1-AP
WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000.
WB analysis of mouse muscle/liver tissues using 15821-1-AP
WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).
WB analysis of mouse heart tissue using 15821-1-AP
WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).
WB analysis of 293 cell using 15821-1-AP
WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control).
WB analysis of HepG2/MCF7 cells using 15821-1-AP
WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).
WB analysis of mouse skeletal muscle using 15821-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 15821-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15821-1-AP
IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug.
IHC staining of human gliomas using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skeletal muscle using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15821-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 15821-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissues, mouse heart tissue, 293 cell, HepG2/MCF7 cells, mouse colon tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue, human skeletal muscle tissue, human brain tissue, human heart tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 71 publications below
IHC	See 12 publications below
IF	See 4 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

15821-1-AP targets PKM1-specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide
Full Name	PKM pyruvate kinase, muscle
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	NM_182470
Gene Symbol	PKM
Gene ID (NCBI)	5315
RRID	AB_2163820
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM1-specific antibody 15821-1-AP	Download protocol
IHC protocol for PKM1-specific antibody 15821-1-AP	Download protocol
IP protocol for PKM1-specific antibody 15821-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC	Nature The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Authors - Christofk Heather R HR KD Validated View Article
human		Nat Immunol NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death. Authors - Di Huang View Article
human	WB,CoIP	Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence Authors - Yinsheng Wu View Article
human	WB	Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. Authors - Chunqing Wang View Article
human	WB	Nat Commun The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Authors - Fang Zheng View Article
human	WB	Nat Commun Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23. Authors - Yao Wei View Article

PKM1-specific Polyclonal antibody

PKM1-specific Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 15821-1-AP

WB analysis using 15821-1-AP

WB analysis using 15821-1-AP

WB analysis using 15821-1-AP

WB analysis of multi-cells/tissue using 15821-1-AP

WB analysis of mouse muscle/liver tissues using 15821-1-AP

WB analysis of mouse heart tissue using 15821-1-AP

WB analysis of 293 cell using 15821-1-AP

WB analysis of HepG2/MCF7 cells using 15821-1-AP

WB analysis of mouse skeletal muscle using 15821-1-AP

WB analysis of mouse brain using 15821-1-AP

IP experiment of mouse brain using 15821-1-AP

IHC staining of human gliomas using 15821-1-AP

IHC staining of human gliomas using 15821-1-AP

IHC staining of human gliomas using 15821-1-AP

IHC staining of mouse heart using 15821-1-AP

IHC staining of mouse heart using 15821-1-AP

IHC staining of human skeletal muscle using 15821-1-AP

IHC staining of human skeletal muscle using 15821-1-AP

IHC staining of human brain using 15821-1-AP

IHC staining of human brain using 15821-1-AP

IHC staining of human heart using 15821-1-AP

IHC staining of human heart using 15821-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence

Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.

The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23.

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