PKC Delta Recombinant antibody, PBS Only

PKC Delta Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241608A2

Cat no : 84261-5-PBS

Synonyms

PRKCD, PKCD, nPKC-delta, MAY1, EC:2.7.11.13

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 84261-5-RR (same clone as 84261-5-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84261-5-RR (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84261-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 84261-5-RR (same clone as 84261-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84261-5-RR (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84261-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84261-5-RR (same clone as 84261-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKCD antibody (84261-5-RR, Clone: 241608A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84261-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HT-1080 using 84261-5-RR (same clone as 84261-5-PBS)

  1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug PRKCD Recombinant Antibody (84261-5-RR, Clone:241608A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84261-5-PBS in a different storage buffer formulation.

• 

  FC experiment of Hela using 84261-5-RR (same clone as 84261-5-PBS)

  1x10^6 Hela cells were intracellularly stained with 0.25 ug PRKCD Recombinant Antibody (84261-5-RR, Clone:241608A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84261-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84261-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84261-5-RR against Human PKC Delta were performed. The affinity constant is 0.437 nM.

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Product Information

The immunogen of 84261-5-PBS is PKC Delta Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PKC Delta fusion protein Ag5473
• Full Name: protein kinase C, delta
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC043350
• Gene Symbol: PKC Delta
• Gene ID (NCBI): 5580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.