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PKC Delta Recombinant antibody

PKC Delta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241608A2

Cat no : 84261-5-RR

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using PKC Delta Recombinant antibody (84261-5-RR)

    WB analysis of NIH/3T3 using 84261-5-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84261-5-RR (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PKC Delta Recombinant antibody (84261-5-RR)

    WB analysis using 84261-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84261-5-RR (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Delta Recombinant antibody (84261-5-RR)

    IF Staining of HeLa using 84261-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRKCD antibody (84261-5-RR, Clone: 241608A2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HT-1080 cells using PKC Delta Recombinant antibody (84261-5-RR)

    FC experiment of HT-1080 using 84261-5-RR

    1x10^6 HT-1080 cells were intracellularly stained with 0.25 ug PRKCD Recombinant Antibody (84261-5-RR, Clone:241608A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Hela cells using PKC Delta Recombinant antibody (84261-5-RR)

    FC experiment of Hela using 84261-5-RR

    1x10^6 Hela cells were intracellularly stained with 0.25 ug PRKCD Recombinant Antibody (84261-5-RR, Clone:241608A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHT-1080 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84261-5-RR is PKC Delta Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PKC Delta fusion protein Ag5473
Full Name protein kinase C, delta
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.

Protocols

Product Specific Protocols
WB protocol for PKC Delta antibody 84261-5-RRDownload protocol
IF protocol for PKC Delta antibody 84261-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols