PICK1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 82919-1-RR (same clone as 82919-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82919-1-PBS in a different storage buffer formulation.

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  WB analysis using 82919-1-RR (same clone as 82919-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82919-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00068-3

  Sandwich ELISA standard curve of MP00068-3, Human PICK1 Recombinant Matched Antibody Pair - PBS only. 82919-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1443. 82919-1-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL

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  Sample test of MP00068-3

  The mean PICK1 concentration was determined to be 9.40 ng/mL in LNCaP cell extract based on a 3.80 mg/mL extract load and 4.08 ng/mL in MCF-7 cell extract based on a 1.5 mg/mL extract load.

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  IHC staining of human stomach cancer using 82919-1-RR (same clone as 82919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82919-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82919-1-RR (same clone as 82919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82919-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82919-1-RR (same clone as 82919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82919-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 82919-1-RR (same clone as 82919-1-PBS)

  1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82919-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00068-2

  Cytometric bead array standard curve of MP00068-2, PICK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82919-3-PBS. Detection antibody: 82919-1-PBS. Standard: Ag1443. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 82919-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82919-1-RR against Human PICK1 were performed. The affinity constant is 0.792 nM.

PICK1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 82919-1-PBS

Clone No.230185B8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Protein kinase C-alpha-binding protein, PRKCABP, PRKCA-binding protein, PRKCA binding protein, PICK

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Product Information

82919-1-PBS targets PICK1 as part of a matched antibody pair:

MP00068-2: 82919-3-PBS capture and 82919-1-PBS detection (validated in Cytometric bead array)

MP00068-3: 82919-2-PBS capture and 82919-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PICK1 fusion protein Ag1443
• Full Name: protein interacting with PRKCA 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC017561
• Gene Symbol: PICK1
• Gene ID (NCBI): 9463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRD5
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.