Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 82919-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human stomach cancer using 82919-1-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 82919-1-RR
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 82919-1-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HEK-293T using 82919-1-RR
1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 82919-1-RR
Biolayer interferometry (BLl) kinetic assays of 82919-1-RR against Human PICK1 were performed. The affinity constant is 0.792 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
LNCaP cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected in
human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected in
HEK-293T cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunohistochemistry (IHC)
IHC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
82919-1-RR targets PICK1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.
Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 82919-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82919-1-RR (PICK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human stomach cancer using 82919-1-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 82919-1-RR
Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 82919-1-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82919-1-RR (PICK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC (INTRA) Figures
FC experiment of HEK-293T using 82919-1-RR
1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human PICK1 (82919-1-RR, Clone:230185B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 82919-1-RR
Biolayer interferometry (BLl) kinetic assays of 82919-1-RR against Human PICK1 were performed. The affinity constant is 0.792 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PICK1 Recombinant antibody
Catalog Number
82919-1-RR
Citations
-
Dilutions
WB : 1:1000-1:8000 IHC : 1:400-1:1600 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, FC (Intra), ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
