CoraLite® Plus 488-conjugated PI3 Kinase p85 Beta Monoclonal antibody

PI3 Kinase p85 Beta Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67644
Clone No.2B3B6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67644 targets PI3 Kinase p85 Beta in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p85 Beta fusion protein Ag14369 Predict reactive species
Full Name phosphoinositide-3-kinase, regulatory subunit 2 (beta)
Calculated Molecular Weight 728 aa, 82 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014170
Gene Symbol PI3 Kinase p85 Beta
Gene ID (NCBI) 5296
RRIDAB_3672955
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00459
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PI3 Kinase p85 Beta antibody CL488-67644Download protocol
FC protocol for CL Plus 488 PI3 Kinase p85 Beta antibody CL488-67644Download protocol
Standard Protocols
Click here to view our Standard Protocols
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