CoraLite® Plus 488-conjugated PI3 Kinase p85 Beta Monoclonal antibody

PI3 Kinase p85 Beta Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B3B6

Cat no : CL488-67644

Synonyms

PI3K p85 β, PI3K p85(beta), PI3K p85β, PIK3R2, p85β

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67644

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PI3 Kinase p85 Beta antibody (CL488-67644, Clone: 2B3B6 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-67644

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pi3 Kinase P85 Beta Monoclonal Antibody (CL488-67644, Clone:2B3B6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67644 targets PI3 Kinase p85 Beta in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p85 Beta fusion protein Ag14369
• Full Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta)
• Calculated Molecular Weight: 728 aa, 82 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC014170
• Gene Symbol: PI3 Kinase p85 Beta
• Gene ID (NCBI): 5296
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317).