CoraLite®555-conjugated PI3 Kinase p110 Beta Monoclonal antibody

PI3 Kinase p110 Beta Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1B2A1

Cat no : CL555-67121

Synonyms

DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3K p110(beta), PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

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Validation Data Gallery

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  FC experiment of HeLa using CL555-67121

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PI3 Kinase p110 Beta (CL555-67121, Clone:1B2A1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67121 targets PI3 Kinase p110 Beta in FC (Intra) applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PI3 Kinase p110 Beta fusion protein Ag17505
• Full Name: phosphoinositide-3-kinase, catalytic, beta polypeptide
• Calculated Molecular Weight: 1070 aa, 123 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC114432
• Gene Symbol: PI3 Kinase p110 Beta
• Gene ID (NCBI): 5291
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002).