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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated PI3 Kinase p110 Beta Polyclonal antibody

PI3 Kinase p110 Beta Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-20584

Synonyms

DKFZp779K1237, p110 BETA, PI3 Kinase p110 Beta, PI3 kinase subunit beta, PI3K, PI3K beta, PI3Kbeta, PI3KCB, PIK3C1, PIK3CB, PtdIns 3 kinase subunit beta

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated PI3 Kinase p110 Beta Polyc (CL555-20584)

    IF Staining of HeLa using CL555-20584

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 PI3 Kinase p110 Beta antibody (CL555-20584) at dilution of 1:100.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-20584 targets PI3 Kinase p110 Beta in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphoinositide-3-kinase, catalytic, beta polypeptide
Calculated Molecular Weight 123 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberNM_006219
Gene Symbol PI3 Kinase p110 Beta
Gene ID (NCBI) 5291
RRIDAB_2919637
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PIK3CB(phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform) is also named as PIK3C1, PI3K-beta, p110beta. The gene encodes a 1070 amino acid protein which belongs to the PI3/PI4-kinase family. Phosphoinositide 3-kinases (PI3Ks) have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. The class I PI3 kinases are heterodimers composed of 110 kDa catalytic subunits that associate with regulatory adaptor proteins. Four class I catalytic subunits have been identified, PIK3CA (p110α), PIK3CB (p110β), PIK3CD (p110δ) and PIK3CG (p110γ)(PMID:19177002). This antibody is specific to PIK3CB.

Protocols

Product Specific Protocols
IF protocol for CL555 PI3 Kinase p110 Beta antibody CL555-20584Download protocol
FC protocol for CL555 PI3 Kinase p110 Beta antibody CL555-20584Download protocol
Standard Protocols
Click here to view our Standard Protocols