CoraLite® Plus 488-conjugated PHLPP Polyclonal antibody

• Featured Product
• KD/KO Validated

PHLPP Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22789

Synonyms

hSCOP, KIAA0606, PHLPP, PHLPP1, PLEKHE1, SCOP

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL488-22789

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PHLPP antibody (CL488-22789) at dilution of 1:400.

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22789 targets PHLPP in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHLPP fusion protein Ag18794
• Full Name: PH domain and leucine rich repeat protein phosphatase
• Calculated Molecular Weight: 1717 aa, 184 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC126277
• Gene Symbol: PHLPP
• Gene ID (NCBI): 23239
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.