PHLPP Polyclonal antibody

• Featured Product
• KD/KO Validated

PHLPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

33

Reviews (1)

Cat no : 22789-1-AP

Synonyms

PLEKHE1, PHLPP1, PH domain-containing family E member 1, PH domain leucine-rich repeat-containing protein phosphatase 1, KIAA0606

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Validation Data Gallery

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  WB analysis of HeLa using 22789-1-AP

  WB result of PHLPP antibody (22789-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPP transfected HeLa cells.

• 

  WB analysis using 22789-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22789-1-AP (PHLPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22789-1-AP

  IP result of anti-PHLPP (IP:22789-1-AP, 4ug; Detection:22789-1-AP 1:600) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human liver using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22789-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22789-1-AP (PHLPP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22789-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHLPP antibody (22789-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 30 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 5 publications below

Product Information

22789-1-AP targets PHLPP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHLPP fusion protein Ag18794
• Full Name: PH domain and leucine rich repeat protein phosphatase
• Calculated Molecular Weight: 1717 aa, 184 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC126277
• Gene Symbol: PHLPP
• Gene ID (NCBI): 23239
• RRID: AB_2750897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHLPP (PH domain leucine-rich-repeats protein phosphatase) belongs to a novel family of Ser/Thr protein phosphatases that consists of PHLPP1 and PHLPP2 isoforms. It has been shown that PHLPP negatively regulates multiple oncogenic pathways by directly dephosphorylating and inactivating key signaling molecules, including Akt, S6K, and RAF1. Observed MW of PHLPP is 185 kDa.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.; Authors - Geethu Emily Thomas ; KD Validated
human,mouse	WB,IHC,IP	Mol Cancer; CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.; Authors - Tao Jiang ; KD Validated
mouse	WB	J Pineal Res; Melatonin increases susceptibility to atrial fibrillation in obesity via Akt signaling impairment in response to lipid overload; Authors - Xinghua Qin
human	WB	EMBO Rep; USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.; Authors - Dana Goldbraikh ; KD Validated
mouse	WB	Cell Mol Immunol; CUL4B negatively regulates Toll-like receptor-triggered proinflammatory responses by repressing Pten transcription.; Authors - Yu Song
mouse	WB	Elife; PHLPP1 counter-regulates STAT1-mediated inflammatory signaling.; Authors - Ksenya Cohen Katsenelson ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!

• Applications: Western Blot
• Cell Tissue Type: SHED cell lines

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