PGM1 Monoclonal antibody, PBS Only

PGM1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G5A8

Cat no : 66105-1-PBS

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

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Validation Data Gallery

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  WB analysis using 66105-1-Ig (same clone as 66105-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100. This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66105-1-Ig (same clone as 66105-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66105-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66105-1-PBS targets PGM1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PGM1 fusion protein Ag7294
• Full Name: phosphoglucomutase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC001756
• Gene Symbol: PGM1
• Gene ID (NCBI): 5236
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.