PGM1 Monoclonal antibody
PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, rabbit, pig
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2G5A8
Cat no : 66105-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MDA-MB-231 cells, HepG2 cells, pig liver tissue, rabbit liver tissue, rat liver tissue, mouse liver tissue, pig heart tissue, rabbit heart tissue |
Positive IHC detected in | human colon tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66105-1-Ig targets PGM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.
Tested Reactivity | human, mouse, rat, rabbit, pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | PGM1 fusion protein Ag7294 |
Full Name | phosphoglucomutase 1 |
Calculated Molecular Weight | 61 kDa |
Observed Molecular Weight | 61 kDa |
GenBank Accession Number | BC001756 |
Gene Symbol | PGM1 |
Gene ID (NCBI) | 5236 |
RRID | AB_2881504 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for PGM1 antibody 66105-1-Ig | Download protocol |
IHC protocol for PGM1 antibody 66105-1-Ig | Download protocol |
IF protocol for PGM1 antibody 66105-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |