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CoraLite®594-conjugated PFKFB3-Specific Polyclonal antibody
PFKFB3-Specific Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-13763
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-13763 targets PFKFB3-Specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PFKFB3-Specific fusion protein Ag4744 |
Full Name | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 |
Calculated Molecular Weight | 520 aa, 60 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | BC040482 |
Gene Symbol | PFKFB3 |
Gene ID (NCBI) | 5209 |
RRID | AB_2919799 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. It synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5. This antibody is specific to PFKFB3.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 PFKFB3-Specific antibody CL594-13763 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |