CoraLite® Plus 488-conjugated PDLIM7 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDLIM7 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10221

Synonyms

LMP1, LIM mineralization protein, ENIGMA

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Validation Data Gallery

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  IF Staining of SKOV-3 using CL488-10221

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite® Plus 488 PDLIM7 antibody (CL488-10221) at dilution of 1:200.

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  FC experiment of HeLa using CL488-10221

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Pdlim7 Polyclonal Antibody (CL488-10221)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: SKOV-3 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10221 targets PDLIM7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDLIM7 fusion protein Ag0264
• Full Name: PDZ and LIM domain 7 (enigma)
• Calculated Molecular Weight: 55/60 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC001093
• Gene Symbol: PDLIM7
• Gene ID (NCBI): 9260
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.