PDLIM7 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDLIM7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (2)

Cat no : 10221-1-AP

Synonyms

LMP1, LIM mineralization protein, ENIGMA

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Validation Data Gallery

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  WB analysis of HeLa using 10221-1-AP

  WB result of PDLIM7 antibody (10221-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

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  WB analysis of SKOV-3 using 10221-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10221-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10221-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10221-1-AP

  IP result of anti-PDLIM7 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:20000) with MCF-7 cells lysate 1000 ug.

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  IP experiment of MCF-7 using 10221-1-AP

  IP result of anti-PDLIM7 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:300) with MCF-7 cells lysate 2000ug.

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  IHC staining of human colon using 10221-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10221-1-AP (PDLIM7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10221-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10221-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of SKOV-3 using 10221-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using PDLIM7 antibody (10221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10221-1-AP targets PDLIM7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDLIM7 fusion protein Ag0264
• Full Name: PDZ and LIM domain 7 (enigma)
• Calculated Molecular Weight: 55/60 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC001093
• Gene Symbol: PDLIM7
• Gene ID (NCBI): 9260
• RRID: AB_2161789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification.; Authors - Alon Abend
human,mouse	WB,IHC,IP	Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
human	IF	J Bone Miner Res; LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity.; Authors - Hehai Pan
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
human	IHC	J Endod; Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.; Authors - Wang Xiaoying X
human	WB	J Orthop Res; LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.; Authors - Hui Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.

• Applications: Western Blot
• Cell Tissue Type: Heart

FH

Joleen (Verified Customer) (06-10-2019)

The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

PDLIM7 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10221-1-AP)