CoraLite® Plus 647 Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recombinant Antibody

PD-1/CD279 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

EH12.2H7

Cat no : CL647-65586

Synonyms

CD279, PD1, PD-1, PD 1, hPD-1

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65586

  1x10^6 untreated or PHA treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (CL647-65586, Clone:EH12.2H7). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-65586

  1x10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (CL647-65586, Clone:EH12.2H7) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (CL647-65586, Clone:EH12.2H7) (black, dashed). Cells were not fixed.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65586 targets PD-1/CD279 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 288 aa, 32 kDa
• GenBank Accession Number: BC074740
• Gene Symbol: PD-1
• Gene ID (NCBI): 5133
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).