Anti-Human PD-1/CD279 (EH12.2H7) Mouse IgG2a Recombinant Antibody

PD-1/CD279 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

EH12.2H7

Cat no : 65586-1-MR

Synonyms

PD-1 (CD279), CD279, PD1, PD-1, PD 1

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Validation Data Gallery

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  FC experiment of human PBMCs using 65586-1-MR

  1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65586-1-MR

  1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). 1x10^6 PHA treated human PBMCs were surface stained with Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L) (blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 65586-1-MR

  1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) (left) or 0.25 ug Anti-Human PD-1/CD279 (65168-1-Ig, Clone: EH12.2H7) (right) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65586-1-MR targets PD-1/CD279 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 288 aa, 32 kDa
• GenBank Accession Number: BC074740
• Gene Symbol: PD-1
• Gene ID (NCBI): 5133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).